Single cell isolation process with laser induced forward transfer

Single cell isolation process with laser induced forward transfer

BACKGROUND A viable single cell is crucial for studies of single cell biology. In this paper, laser-induced forward transfer (LIFT) was used to isolate individual cell with a closed chamber designed to avoid contamination and maintain humidity. Hela cells were used to study the impact of laser pulse energy, laser spot size, sacrificed layer thickness and working distance. The size distribution,...

High Speed Photography of Laser Induced Forward Transfer (LIFT) of Single and Double-layered Transfer Layers for Single Cell Transfer

Bioprinting technologies allow the construction of tissue-like structures from different cell populations. Fundamental research on the influence of the cell micro-environment requires printing of single cells in specific patterns on a microscopic scale. Single cell printing of living cells has been performed by nozzle based techniques or micro-pipetting, and laser-induced forward transfer (LIFT...

Laser-induced Forward Transfer of Ag Nanopaste

Over the past decade, there has been much development of non-lithographic methods(1-3) for printing metallic inks or other functional materials. Many of these processes such as inkjet(3) and laser-induced forward transfer (LIFT)(4) have become increasingly popular as interest in printable electronics and maskless patterning has grown. These additive manufacturing processes are inexpensive, envi...

Laser-Induced Forward Transfer of Pure Metals

Laser Induced Forward Transfer for Materials Patterning

Today, a strong research effort is directed towards the development and integration of new materials, especially of polymers and biomolecules which are essential in pharmaceutical, bioengineering, and sensing applications. In fact, patterning of organic and inorganic materials, in particular enabling precise positioning of different materials with nano and micro scale resolution over large area...